Journal: Frontiers in Immunology

Article Title: Characterization of the Immunologic Phenotype of Dendritic Cells Infected With Herpes Simplex Virus 1

doi: 10.3389/fimmu.2022.931740

Figure Lengend Snippet: The mutated strain M6 produced a more effective immune response than the wild-type strain. Bodyweight (A) and survival rate (B) of mice infected with the attenuated HSV-1 mutant M6 (circle) and the wild-type strain (square) via nasal spray. (C) Viral loads in the nose, brain, spinal cord, trigeminal ganglion, and inguinal lymph node after infection with M6 (circle) and WT (square), as determined by RT–qPCR. (D) The HSV-1 neutralizing antibody titer in mice infected with M6 (black square), WT (white square), and Mock (gray square). (E) The ELISpot responses show IFN-γ- and IL-4-secreting cells among splenic lymphocytes after infection with M6 (black square), WT (white square), and Mock (gray square). Bodyweight (F) and survival rate (G) of mice immunized with M6 (circle) and WT (square) after wild-type viral challenge. The data are shown as the mean ± SEM based on data from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NS, no significance.

Article Snippet: Murine bone marrow (BM) cultures were initiated at 1×10 6 cells/mL in Roswell Park Memorial Institute (RPMI) 1640 complete medium (Cat#R8758, Sigma) containing 20 ng/mL GM-CSF (HY-P7361, MCE, USA) and 10 ng/mL IL-4 (HY-P70644, MCE, USA).

Techniques: Produced, Infection, Mutagenesis, Quantitative RT-PCR, Enzyme-linked Immunospot

Journal: Ecotoxicology and environmental safety

Article Title: Dibutyl phthalate promotes central precocious puberty through primordial follicle activation by downregulating BMP15.

doi: 10.1016/j.ecoenv.2025.118460

Figure Lengend Snippet: Fig. 4. DBP exposure reduced ovarian BMP15 expression and serum AMH concentration. (a, b) Ovarian BMP15 protein expression level in CON and DBP group. (c) Serum AMH concentration in CON and DBP group. * * P < 0.01.

Article Snippet: Therefore, the experimental groups were control group, MBP group, MBP plus BMP15 (HY-P72426, MedChemExpress) group and MBP plus DHC group (n = 3).

Techniques: Expressing, Concentration Assay

Journal: Ecotoxicology and environmental safety

Article Title: Dibutyl phthalate promotes central precocious puberty through primordial follicle activation by downregulating BMP15.

doi: 10.1016/j.ecoenv.2025.118460

Figure Lengend Snippet: Fig. 5. MBP exposure impair ovarian BMP15 and AMH expression. (a) Protein bands of ovary after MBP treatment by western blot analysis. Protein expression of BMP15 (b), SOX9 (d) and phosphorylation level of p38-MAPK (c). (e) Gene expression of AMH after MBP exposure. * P < 0.05, * * P < 0.01, * ** P < 0.001.

Article Snippet: Therefore, the experimental groups were control group, MBP group, MBP plus BMP15 (HY-P72426, MedChemExpress) group and MBP plus DHC group (n = 3).

Techniques: Expressing, Western Blot, Phospho-proteomics, Gene Expression

Journal: Ecotoxicology and environmental safety

Article Title: Dibutyl phthalate promotes central precocious puberty through primordial follicle activation by downregulating BMP15.

doi: 10.1016/j.ecoenv.2025.118460

Figure Lengend Snippet: Fig. 6. BMP15 mediate MBP downregulation of AMH expression. (a) Protein bands of ovary after MBP plus BMP15 treatment by western blot analysis. Phos phorylation level of p38-MAPK (b) and protein expression of SOX9 (c) and. (d) Gene expression of AMH after treatment. * P < 0.05, * * P < 0.01.

Article Snippet: Therefore, the experimental groups were control group, MBP group, MBP plus BMP15 (HY-P72426, MedChemExpress) group and MBP plus DHC group (n = 3).

Techniques: Expressing, Western Blot, Gene Expression

Journal: Ecotoxicology and environmental safety

Article Title: Dibutyl phthalate promotes central precocious puberty through primordial follicle activation by downregulating BMP15.

doi: 10.1016/j.ecoenv.2025.118460

Figure Lengend Snippet: Fig. 7. MBP downregulate AMH via BMP15/p38-MAPK pathway. (a) Protein bands of ovary after MBP plus dehydrocorydaline treatment by western blot analysis. Protein expression of BMP15 (b) and SOX9 (c). (d) Gene expression of AMH after treatment. * P < 0.05, * * P < 0.01.

Article Snippet: Therefore, the experimental groups were control group, MBP group, MBP plus BMP15 (HY-P72426, MedChemExpress) group and MBP plus DHC group (n = 3).

Techniques: Western Blot, Expressing, Gene Expression